Modulation Of Anti-Tumor Immune Response By Tgf-ÎTM-Inducible Early Gene 1 (tieg1)

........................................................................................... 70 Autobiographical Statement ................................................................... 72 
































 
 vii
 LIST OF FIGURES Figure 1.1 The Her-2 oncoprotein ......................................................... 4 Figure 1.2 DNA vaccination ................................................................. 5 Figure 1.3 The humoral immune response .............................................. 8 Figure 1.4 The cellular immune response .............................................. 10 Figure 1.5 Immunosuppressive mechanisms .......................................... 12 Figure 1.6 TGF-β induction of Foxp3 via TIEG1 in naïve CD4+ T cells ......... 18 Figure 3.1 Generation and genotyping of TIEG1-/mouse line .................... 26 Figure 3.2 Allogeneic Splenocyte Immunization ....................................... 31 Figure 3.3 Immunization with Her-2-Expressing Tumor Cells ...................... 34 Figure 3.4 Her-2 DNA Vaccination ........................................................ 37 Figure 3.5 IgG Subclass in Her-2 DNA Vaccination .................................. 39 Figure 3.6 In Vivo B Cell Numbers and In Vitro Proliferation ....................... 40 Figure 3.7 Natural Treg and Myeloid-Derived Suppressor Cells .................. 42 Figure 3.8 Tumor Growth .................................................................... 46 Figure 3.9 TIEG1-/-Selection ............................................................... 48